mcherry激發(fā)波長是多少？
mCherry是一種來自于蘑菇珊瑚（mushroom coral）的紅色熒光蛋白，常有于標(biāo)記和示蹤某些分子和細(xì)胞組分。相對于其他熒光，mCherry的好處在于它的顏色和應(yīng)用最多的綠色熒光蛋白（GFP）能進(jìn)行共同標(biāo)記，并且mCherry相對于其他單體熒光蛋白來說也具有卓越的光穩(wěn)定型。

mcherry激發(fā)波長是多少？

mCherry是一種來自于蘑菇珊瑚（mushroom coral）的紅色熒光蛋白，常有于標(biāo)記和示蹤某些分子和細(xì)胞組分。相對于其他熒光，mcherry的好處在于它的顏色和應(yīng)用最多的GFP綠色熒光蛋白綠色熒光蛋白（GFP）能進(jìn)行共同標(biāo)記，并且mcherry相對于其他單體熒光蛋白來說也具有卓越的光穩(wěn)定型。

mcherry紅色熒光蛋白
吸收和發(fā)射波長：

mcherry的更大吸收/發(fā)射峰分別位于587nm和610nm，對光致漂白耐受，熒光非常穩(wěn)定。

mcherry紅色熒光蛋白

常見應(yīng)用：
?mCherry常用于與目的基因組成融合蛋白以及通過IRES或2A與感興趣的蛋白共表達(dá)；
?啟動(dòng)子活性研究；
?熒光共振能量轉(zhuǎn)移(fluorescence resonance energy transfer，F(xiàn)RET）和其他定量實(shí)驗(yàn)；
?標(biāo)記細(xì)胞或者分子，進(jìn)行示蹤實(shí)驗(yàn)；

mcherry紅色熒光蛋白

 mCherry is a fluorophore (a fluorescent protein) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cell components. mCherry and the majority of red fluorescent proteins derive from a protein isolated from Discosoma sp., while other fluorescent proteins in the green range are often variants of GFP from Aequorea victoria.

 
m前綴的意義：
mCherry的m為monmer單體的縮寫，表示mCherry熒光蛋白的形式為單體，這在很多實(shí)驗(yàn)設(shè)計(jì)中非常重要，比如與目的標(biāo)記基因組成融合蛋白時(shí)。

成熟時(shí)間：
mCherry具有較快的成熟速度，t0.5為15分鐘，這在一些需要做出快速反應(yīng)的實(shí)驗(yàn)非常重要。比如啟動(dòng)子活性報(bào)告系統(tǒng)等。

如何觀察和篩選mcherry在植物中的表達(dá)？

mcherry紅色熒光蛋白的激發(fā)波長和發(fā)射波長

實(shí)驗(yàn)室常見熒光蛋白的激發(fā)波長和發(fā)射波長

紫外熒光蛋白UV Proteins

藍(lán)色熒光蛋白Blue Proteins

青色熒光蛋白Cyan Proteins

綠色熒光蛋白Green Proteins

黃色熒光蛋白Yellow Proteins

桔色熒光蛋白Orange Proteins

紅色熒光蛋白R(shí)ed Proteins

遠(yuǎn)紅熒光蛋白Far Red Proteins

近紅熒光蛋白Near IR Proteins

Sapphire-type Proteins

Red Fluorescent Proteins

Red fluorescent proteins (RFP) can be imaged on existing confocal or widefield microscopes, and they also have more penetrating power. The excitation and emission maxima of RFP are 558nm and 583 nm, respectively.

The use of RFP, however, has been hampered with several issues. RFP is an obligate tetramer - thus, it forms large aggregates inside cells. This makes the use to RFP to report the location of a protein severely limited.

Although GFP can successfully fuse with several hundreds of proteins, RFP-conjugated proteins are often toxic. Some variants of RFP have overcome these limitations. For example, DsRed2 fluorescent protein does not form aggregates and has reduced toxicity, while another variant of RFP (known as RedStar) has increased brightness and maturation rate.